Abstract: Effect of LY294002 on Subcutaneous Human Endometrial Carcinoma Xenografts in Nude MiceRuixia GUO, Ruifang ZHANG, XinyanWANG, Huirong SHI, Yuhuan QIAOCorrespondence to: Ruixia GUO, E-mail: tjmgrx@yahoo.com.cnThe Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaThis work was supported by a grant from Science and Technology Innovation Fund of He'nan Province (Grant no. 2010HASTTTDD5)Abstract Objective: To investigate the effects of the signal pathway inhibitor LY294002 on cell proliferation, apoptosis, andp-AKT expression in endometrial carcinoma xenografts. Methods: Human endometrial carcinoma Ishikawa cells were cultured in vi-tro. The models of xenografted tumor were established through the subcutaneous inoculation of 12 nude mice, which were then random-ly divided into two groups for intraperitoneal injections with normal saline ( NS ) and LY294002. The anti-tumor effect was evaluatedby measuring tumor volume and tumor growth. The histopathologic changes in tumor specimens were observed using hematoxylin andeosin ( HE ) staining, and the p-AKT expression levels were detected by immunohistochemistry. Results: 1) Compared with the controlgroup, the increase in transplanting tumor volume in the treated group was obvious ( t = 4.082, P = 0.005 ). The inhibition tumor ratewas 32.49% ± 16.39%. 2 ) HE staining showed that apoptosis and necrosis of the tumor cells were significantly increased in the treatedgroup. Immunohistochemistry results demonstrated that the p-AKT expression in the treated group was lower than that in the controlgroup ( χ2= 6.133, P < 0.05 ). Conclusion: The pathway inhibitor LY294002 inhibits the growth of subcutaneous human endometrialcarcinoma xenografts in nude mice and induces cell apoptosis.Keywords Nude mice; Endometrial carcinoma; LY294002; Phosphorylated AKT ( p-AKT )